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货号: TPFL0020
货期: 现货
价格: 1306.28
所属分类：
靶点: Ki67
应用： IHC-P, Flow Cyt (Intra), mIHC, ICC/IF, WB
反应性 ： Mouse, Rat, Human
宿主物种 ： Rabbit
MW(CalcμLated) ： 358 kDa
MW(Observed)： 124 kDa; 359 kDa
同种型-Isotype ： IgG
隐藏域元素占位
详细信息
推荐稀释比 ： mIHC: 1/200；ICC/IF: 1/250；IHC-P: 1/200；Flow Cyt (Intra): 1/1000
组成 ： pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
纯化工艺 ： Affinity purification Protein A
储存 ： Form: Liquid，Shipping condition: Blue Ice，Short-term storage (1–2 weeks): +4°C，Long-term storage: -20°C，Aliquot information:，Aliquot upon delivery，Storage note: Avoid freeze-thaw cycles.
克隆性 ： Monoclonal
克隆号 ： SP6
抗原&靶点信息
特异性 ： <p>Ki67 is mainly expressed in proliferating cells. For normal tissue samples (e.g., liver, kidney), no staining may be typically observed due to low level of proliferation and little expression of Ki67&#46; For malignant tissue samples (e.g., colon carcinoma, breast carcinoma), it is more easily to find Ki67 in the proliferating cells of these tissues (PMID: 6206131, 10653597, 34183782&#41;.</p><p><strong>FURTHER INFORMATION ON SPECIFICITY</strong> <strong>(Chinese Version)&nbsp;</strong>available under the Product Protocol section.</p>
基因名称 ： MKI67
蛋白名称 ： Proliferation marker protein Ki-67
别名 ： Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67
背景：
Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis (PubMed : 27362226, PubMed : 32879492, PubMed : 35513709, PubMed : 39153474). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed : 27362226). During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface (PubMed : 27362226). The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier : the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed : 27362226). During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space (PubMed : 32879492, PubMed : 39153474). Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes (PubMed : 39153474). Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation (PubMed : 28935370, PubMed : 39153474). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed : 10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome (PubMed : 24867636).
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