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推荐稀释比 ： WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
组成 ： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺 ： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存 ： -15°C to -25°C/1 year(Do not lower than -25°C
浓度 ： 1 mg/ml
克隆性 ： Polyclonal
抗原&靶点信息
特异性 ： V-ATPase D1 Polyclonal Antibody detects endogenous levels of V-ATPase D1 protein.
基因名称 ： ATP6V0D1
蛋白名称 ： V-type proton ATPase subunit d 1
别名 ： ATP6V0D1;ATP6D;VPATPD;V-type proton ATPase subunit d 1;V-ATPase subunit d 1;32 kDa accessory protein;V-ATPase 40 kDa accessory protein;V-ATPase AC39 subunit;p39;Vacuolar proton pump subunit d 1
背景：
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [pro
细胞定位 ： Membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Localizes to centrosome and the base of the cilium. .
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