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货号: TPFL0051
货期: 现货
所属分类：
靶点: NF-κB p65
应用： W, W-F, W-S, IP, IF-IC, FC-FP
反应性 ： H M R Hm Mk Pg
宿主物种 ： Rabbit
MW(Observed)： 65
同种型-Isotype ： IgG
隐藏域元素占位
详细信息
推荐稀释比 ： Application Dilution Western Blotting 1:1000 Fluorescent Western 1:1000 Simple Western™ 1:10 - 1:50 Immunoprecipitation 1:50 Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized) 1:800 - 1:3200
来源 ： Rabbit
纯化工艺 ： Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65.
储存 ： Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 76778 .
克隆性 ： Monoclonal
克隆号 ： 93H1
抗原&靶点信息
特异性 ： Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit Monoclonal Antibody detects NF-κB p65 only when phosphorylated at Ser536. This antibody does not cross-react with the p50 subunit or other related proteins. Non-specific labeling may be observed by immunofluorescence in astrocytes. Species predicted to react based on 100% sequence homology Dog
背景：
Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).
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