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货号: TPFL0032
货期: 现货
价格: 5549.43
所属分类：
靶点: CD3 epsilon
应用： IHC-P, WB, IHC-Fr, Flow Cyt (Intra), mIHC, IP
反应性 ： Mouse, Rat, Human
宿主物种 ： Rabbit
同种型-Isotype ： IgG
隐藏域元素占位
详细信息
推荐稀释比 ： mIHC: 1/2000；IHC-P: 1/2000；IP: 1/30；WB: 1/1000；IHC-Fr: 1/500；Flow Cyt (Intra): 1/500
组成 ： pH: 7.5 Preservative: 0.05% Sodium azide Constituents: PBS
纯化工艺 ： Affinity purification Protein A
储存 ： Form: Liquid，Shipping condition: Blue Ice，Short-term storage (1–2 weeks): +4°C，Long-term storage: -20°C，Aliquot information:，Aliquot upon delivery，Storage note: Avoid freeze-thaw cycles.
克隆性 ： Monoclonal
克隆号 ： CAL57
抗原&靶点信息
基因名称 ： CD3E
蛋白名称 ： T-cell surface glycoprotein CD3 epsilon chain
别名 ： CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
背景：
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed : 15294938, PubMed : 15546002, PubMed : 2470098, PubMed : 40592325, PubMed : 8490660). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed : 2470098, PubMed : 40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed : 2470098, PubMed : 40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098, PubMed : 40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (By similarity). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (By similarity). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (PubMed : 38614099). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 12110186, PubMed : 38614099).
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