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推荐稀释比 ： WB 1:500-2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.
组成 ： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺 ： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存 ： -15°C to -25°C/1 year(Do not lower than -25°C
浓度 ： 1 mg/ml
克隆性 ： Polyclonal
抗原&靶点信息
特异性 ： eIF4G Polyclonal Antibody detects endogenous levels of eIF4G protein.
基因名称 ： EIF4G1
蛋白名称 ： Eukaryotic translation initiation factor 4 gamma 1
别名 ： EIF4G1;EIF4F;EIF4G;EIF4GI;Eukaryotic translation initiation factor 4 gamma 1;eIF-4-gamma 1;eIF-4G 1;eIF-4G1;p220
背景：
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010],
细胞定位 ： Cytoplasm, Stress granule .
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