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推荐稀释比 ： WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.
组成 ： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
纯化工艺 ： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存 ： -15°C to -25°C/1 year(Do not lower than -25°C
浓度 ： 1 mg/ml
克隆性 ： Polyclonal
抗原&靶点信息
特异性 ： DNA pol ι Polyclonal Antibody detects endogenous levels of DNA pol ι protein.
基因名称 ： POLI
蛋白名称 ： DNA polymerase iota
别名 ： POLI;RAD30B;DNA polymerase iota;Eta2;RAD30 homolog B
背景：
catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity). Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,
细胞定位 ： Nucleus . Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage. .
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